All posts by Barbara Payne

Inhaled nitric oxide looks safe as agent to fight bacteria

Our bodies’ immune systems naturally produce nitric oxide (NO).  It’s interesting to learn that NO is also known as a “potent anti-microbial agent against bacteria, viruses, fungi, and parasites.” Maybe that’s because up til recently no one’s tested how safe it is to give to humans at levels that would actually kill microbes.

But now a new study has proved NO is indeed safe to use in otherwise healthy adult human beings. According to an item in Health Canal about NO as an antibacterial agent, the study, reported in a recent edition of the Journal of Cystic Fibrosis, says that volunteers “show no adverse effects associated with therapeutic antimicrobial concentrations of nitric oxide” inhaled as directed over a period of a week. The study was conducted through University of British Columbia Faculty of Medicine and Vancouver Coastal Health Research Institute.

All the test has done is prove it’s safe to use NO inhaled according to that protocol. Now the real work begins. Researchers will likely choose to focus first on seeking out and developing a way to use inhaled NO to treat cystic fibrosis and tuberculosis, both resistant pulmonary infections. Another study reported in New England Journal of Medicine showed that inhaled NO positively affected certain measures in people with acute lung injury, but so far it hasn’t demonstrated that anyone will live longer. More tests are needed.

Reminds me of the biography of President James Garfield I just finished. Garfield survived two months after his assassin’s bullet entered his body. This was at a time when Lister had proven the effectiveness of practicing antisepsis, European doctors were all using it, and American doctors dismissed it as poppycock. Garfield suffered terribly after his doctors repeatedly inserted unwashed, unsterilized fingers and probes into his wound. His body eventually totally encapsulated the bullet, which hadn’t hit any vital organs. He could easily have survived his wound, but he died an agonizing long-drawn-out death from all the infections his doctors had introduced.

Who knows who will live one day when all the amazing discoveries we are making at this time are finally translated into useable therapies.

Growing bone outside the body with stem cells from fat

English: Fat Stem Cells
English: Fat Stem Cells (Photo credit: Wikipedia)

Up til now stem cells have generally been injected or otherwise inserted into living tissue to get them to grow into specific types of organs or other tissue. Now scientists in the UK in collaboration with an Israeli research team have managed to grow human bone in the lab with stem cells from fat tissue. They’ve already successfully implanted a piece of lab-grown human bone into a rat’s leg, where it joined nicely with the creature’s existing bone.

The researchers use scans of the damaged bone to construct a gel-like scaffold that shows the stem cells how to grow into the shape of the needed replacement. Then the mold of stem cells is turned into actual bone in a special machine called a bioreactor that provides the conditions needed for this miracle to take place.

The bone grown from stem cells could theoretically be used to replace damaged or missing bone—for example in repairing a cleft palate. They mention using it to fix bones that have been crushed or otherwise mangled in accidents.

I suspect that once this process is perfected, far down the road, doctors may eventually be able to use it to construct replacement bone for arthritic hips and knees. Too bad it will be long after I and my arthritic relatives will be around to have any need for it.

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Blood transfusion issues: plasma expanders bring NO to the rescue

Goalfinder Blood-plasma
Goalfinder Blood-plasma (Photo credit: gfinder)

Blood transfusions often cause complications that cost the American healthcare system—to the tune of $17 billion dollars in total since they started being used, according to the UC San Diego Technology Transfer Office.

Researchers there have shown that expanding the viscosity of blood plasma increases pressure on blood vessel walls and leads the body to produce more nitric oxide (NO). More NO improves production and distribution of oxygen as well as allowing tissues to consume less oxygen. The whole package may obviate some of the difficulties of blood transfusions and even “lessen the need of a transfusion until blood losses lower the red blood cell concentration to one-quarter of normal, reducing hematocrit to approximately 12%.”

“Examples of NO producing plasma expanders include polyethylene glycol (PEG), PEG-albumin (human serum albumin), PEG-hemoglobulin, and long polymers such as dextran, poly-lactic acid, starch.”

These expanders all sound like well-understood  products. So score one for UC San Diego for discovering a gentlerEnhanced by Zemanta, less invasive way to help victims who need acute treatment for fluid and/or blood loss due to hypovolemia, anemia, hemorrhagic shock, surgical procedures, low flow rates, and other conditions.

Fractured sleep in hospitals hinders healing

Hospital  We all know it’s painful to be awakened multiple times in the night while we’re in the hospital. Surprise. Now there’s a quantified set of stats to prove the crummy quality of sleep in the hospital, beyond being just uncomfortable, is actually damaging to our health.

 

Both the levels and the types of hospital night noise were measured in this study and found to seriously disrupt the brain activity and cardiovascular function of patients who are there trying to heal. And that’s not even counting all the times they come into your room on purpose, turn on the lights and wake you up to poke, prod and peer at you.

This study results were conclusive enough that the researchers recommend immediate efforts to decrease noise in existing medical care facilities and ensure that new facilities are built to respect this new understanding of the importance of sleep for recovery.

 

 

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Even accepted targeted lung cancer therapies can miss the point

When a 61-year-old woman died recently after having received an accepted targeted treatment—known as stereotactic body-radiation therapy—doctors had to start re-thinking both the radiation dose and the treatment itself.

Non-small-cell lung carcinoma
Non-small-cell lung carcinoma (Photo credit: Wikipedia)

Months after she’d received the treatment for early-stage adenocarcinoma, a type of non-small cell lung cancer, the woman came back suffering. Doctors found not only that her cancer had metastasized but also that a large portion of healthy tissue in her airway had been destroyed by the radiation.  They then treated her with chemotherapy rather than radiation, but she died anyway a few months later.

Despite doctors following accepted protocol, the patient died. It took courage to report this case, according to the US News and Health article. Even though this incident is about a single patient, having it on the record can help other doctors think more carefully about how and how much to use this increasingly popular therapy.

Let us hope physicians are all as brave and forthcoming if and when they discover dangerous or devastating side effects from the new nanoparticle-based targeted cancer therapy techniques.

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News in stem cell, nano, NO and other research

Happy to report on a number of small but significant developments in biomed research as reported in a recent MedPage Today.

Cell biology
Cell biology (Photo credit: Arenamontanus)

Regrowing heart tissue using bone marrow

stem cells after heart attacks. Healthy donors’ cells seem to work better than using the patient’s own compromised stem cells. Makes sense—if the heart is damaged, doesn’t seem right that cells from it would be okay. But of course it only looks obvious once we’ve actually learned it.

Fighting infection by controlling production of nitric oxide (NO). Silicate powder interacts with light to release NO and kill gram-negative bacteria without harming the host. Another useful partner for NO—and another good reason to get out and enjoy the sun.

Saving lives by preventing organ fibrosis (scarring). Peptide from collagen has prevented scarring in human skin samples and in lungs and skin of mice. It even reversed fibrosis that had already begun in mouse lungs. Wonder if they’ll ever be able to use it for COPD and emphysema victims.

Minimizing damage to healthy tissue by using nano-constructed cages for targeting and delivering drugs to specific cells. They’ve got the concept but need to work on the execution—e.g., controlling the porosity of the cage so the loaded drug doesn’t leak out before it reaches its target.

So much promise. My imagination goes wild with visions of a world with so much less suffering.

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Surprise partners: Nitric oxide and H2S…What else don’t we know?

Blood_Vessels
Blood_Vessels (Photo credit: shoebappa)

The trouble with some of the miracles of science is that we discover some wonderful substance and start manipulating it in the belief we know what we’re doing, only to find out at some later date that the substance in question—in this case, nitric oxide—does not, in fact, perform its magic all by itself.

A recent study reports that hydrogen sulfide (H2S), which was thought to perform on its own certain functions similar to those of nitric oxide (NO), is actually a partner with NO in such actions as growing new blood vessels and relaxing existing ones. The authors of the study were Greek and American scientists, and the report is published in the Proceedings of the National Academy of Sciences of the United States of America.

Adoption of Genetically Engineered Crops in th...
Adoption of Genetically Engineered Crops in the U.S. HT = herbicide tolerance. BT = insect resistance. (Photo credit: Wikipedia)

The only conclusion I can reach is that these researchers were surprised by this discovery. Which makes me wonder. How can we use nanotechnology for a myriad of purposes and gaily go about genetically modifying foods and so on without having done enough safety studies? What surprise “partnerships” might we be missing/ignoring? And if we’re missing something, anything, what long-term effects will, for example, the genetically modified foods have on the nutrition—and therefore growth and health—of the animals and humans consuming them?

I served recently on a small panel of ordinary citizens being questioned by food industry representatives. The topic was attitudes about food safety and food labeling. What kind of labels did we think would make us feel confident about a food? I ask you: if you read “This is really good for you!” on a package, how much do you believe that? How often do you trust that “free range” really means the chickens didn’t spend most of their lives crammed together on top of each other in cages? And does “organic” broccoli mean they used compost from the kitchen in the dirt but still sprayed the hell out of it with pesticides? The truth is often a crapshoot

Some panelists thought they’d trust a source of foods–like Trader Joe’s or Whole Foods–more than they’d trust the marketing language of the manufacturer. The assumption being that these large, consumer-friendly stores that talk big about wholesome and healthy have actually done some serious investigating before they decided to carry a certain brand.

When it comes to nanotech in medicine, I’m sure that if a medicine could be nano-power-injected in me that would save my life—even for a while—I’d say hurry up and shoot, man. But it’s a different story when we talk about using it to fight cancer in a small child where we don’t know what the long-term consequences may be of nanoparticles injected into the body.

No Luddites here. Thank God for every exciting step forward in science—and equal gratitude for those who urge balance and caution.

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Nanoparticles can damage plant DNA

We eat radishes. "Radish Roll" (Photo credit: ulterior epicure)

It’s not good news, but it’s also not unexpected. Recent experiments with adding engineered nanoparticles to plants’ diets determined that the plants started gradually shriveling up and dying.

Specifically, the plants being experimented with were radishes and two ryegrass ground covers that grazing animals commonly eat.  Researchers at National Institute of Standards and Technology (NIST) and the University of Massachusetts Amherst (UMass) wanted to learn:

…whether nanosizing cupric oxide [a reactive chemical that removes electrons from other compounds] made the generation and accumulation of DNA lesions more or less likely in plants. If the former, the researchers also wanted to find out if nanosizing had any substantial effects on plant growth and health.

They found out. The radishes absorbed twice as much cupric oxide and developed twice as many DNA lesions when the mineral came in smaller nanoparticles versus those bigger than 100 nanometers. And the results on the radish seedlings were definitively destructive.

Although the DNA of the two ryegrasses was not as dramatically affected, in all three plant species, growth of both roots and shoots was significantly stunted. Next up for these researchers will be similar testing with “titanium dioxide nanoparticles — such as those used in many sunscreens — on rice plants.”

This report’s conclusions stick strictly to the science and don’t project anything about how the effects of this experiment might apply to human beings being injected or otherwise treated with medicines or protocols involving nano-sized particles. It’s reassuring, at least, to know that high-level researchers are working to test the safety of nanoparticles for living systems. Let’s hope this series of experiments is the first of many that will lead to new, strict standards for nano-sized development.

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Marijuana-type receptors and nitric oxide for spinal cord injuries?

Day 46 - West Midlands Police - Cannabis Drugs...
Day 46 - West Midlands Police - Cannabis Drugs Raid (Photo credit: West Midlands Police)

Some of the oldest medicinal herbs in the world are still being found effective for some treatments for human afflictions. A study in Switzerland, written up  in the April 11, 2012 edition of The Journal of Neuroscience, says a marijuana-like substance works well with nitric oxide to both suppress inhibitors and stimulate activity of motor neurons in the spinal cord.

I’m tempted to conclude that this means something like having these endocannabinoids (a marijuana-like substance) inserted into the body’s neural networks—the authors don’t discuss whether smoking marijuana might produce the same effect—creates a partnership with nitric oxide that promotes nerve activity and could potentially lead to some degree of movement in at least some patients with spinal cord injuries.

But since the abstract doesn’t at any point translate its medical-speak into a recognizable potential benefit to human beings, I’m only guessing. It’s an exciting prospect even if I’ve got it only partially right.

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Talk about magic–nanotechnology guiding stem cells

As baby boomers age, people are also living longer. Naturally they hope to also have improved quality of life—without paying their entire retirement savings for it.

What if we could affordably regenerate blood vessels, bones, cartilage, and even organs? Stem cell research is doggedly pursuing these magical goals, and now nanotechnology is promising to team up to help direct those efforts.

According to a recent presentation at the American Chemical Society (ACS), scientists are beginning to understand biological pathways more deeply. And now nanotech chemistry is coming along to help them implement new ways of doing things. Experts are developing nanotechnology strategies to use “supramolecular self-assembly” to create noodle-like “nanoscale filaments, virus-like objects, or cell-like microcapsules” that can act as scaffolding for building regeneration processes.

The report goes on to say these noodle “gels” can be used with stem cell therapies to help treat spinal cord injury and Parkinson’s disease, to promote rapid growth of blood vessels for heart attacks or diseased arteries, and to facilitate regenerating bone and cartilage.

The patient’s right hip joint replaced by a me...
The patient’s right hip joint replaced by a metal head and a plastic cup. (Photo credit: Wikipedia)

As someone who’s looking at another hip replacement in the near future, this makes me want to say, “Hell, no,” and refuse to let them do the current barbaric procedure. This sawing-and-cutting-out-your-bones thing is so 18th century. Would I ask any questions about whether those nanoparticles could hurt me? Not at my age.

Wonder if I can hold out long enough for this promising tech to bear fruit?

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